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We also calculate the carrier tunneling time for both samples using a modified form of the semiclassical Wentzel Kramers Brillouin (WKB) approximation [26 28].
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Total RNAs were isolated from both samples using an RNeasy mini kit (Qiagen) after 10-hour infection.
FC data was acquired for both samples using an LSR II cytometer (BD Immunocytometry Systems).
In addition, motor and functional recovery patterns were compared between both patient samples, using a generalized linear mixed methods model (GLIMMIX).
Hierarchical clustering was performed on both probes and samples using a Pearson centered similarity measure.
Next, four paths, parallel to each other, were melted on both sides of the samples using a laser beam.
XTEM was carried out on both the 900°C PDA samples using a JEOL 2000FX operated at 200 kV.
DNA was extracted from both leaf and cambium samples using a modified cetyltrimethylammonium bromide (CTAB) method (Cullings, 1992).
The HER2 amplification was assessed on both histological and cytological samples using a Spectrum Green fluorophore-labeled α-satellite DNA probe for chromosome 17 (Chr17) and a Spectrum Orange fluorophore-labeled DNA probe for the HER2 gene locus (Vysis PathVysion HER-2 DNA Probe Kit, Vysis-Abbott, Wiesbaden, Germany).
HTT was detected in both HD and control CSF samples using a pan-HTT antibody raised against the first 17 amino-acid residues at the N terminus of HTT (N17 antibody, Sigma-Aldrich).
DNA was extracted from both parent and nestling blood samples using a chelex extraction method (Walsh et al. 1991).
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