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Quantitative RT-PCR showed successful HOXB7 downregulation in both samples on day 1 following transfection, while scrambled siRNA did not show this downregulation.
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Pre-immune serum samples collected on day 0 were tested alongside serum samples collected on day 42 in the HAI assay (see above) using both the wild-type rHA and SDV rHA as the test antigens.
Viral load was detected in nasal wash samples on day 2 in both groups [ 6].
The percentage of PF (30 to 40 μm) in tissue samples on day seven of culture in both, fresh and frozen-thawed, ovarian tissue samples was used to assess the cryopreservation outcome.
The samples on day 2 and day 3 will be taken during the hypothermic state.
Blood samples on day 2 were taken before study drug administration.
The sample on Day 1 was taken before the start of gonadotrophin administration and the samples on Day 6 and last stimulation day were collected at least 8 h after the previous gonadotrophin dose.
Blood samples on day 0, 3, 7 and 14 were analysed for serum selenium concentration and glutathion peroxidase activity.
To assess serum PSA, investigators drew samples on day 1 and at months 3, 6, 9, and 12.
These data suggest that two-day sampling could not be necessary, once single plasma samples on Day 0 may provide endogenous normalization.
Involution was studied by removing pups from their mother on day 17 of lactation and obtaining samples on day 4 or 8 after weaning.
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