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where n is the number of regions and refers to diversity estimates in which both samples in each pair are from region i.
The second category consists of genes/isoforms in which expression of both samples in each group was up-regulated or down-regulated two-fold or greater between control SFs and RASFs.
The top 10 up- and down-regulated genes, along with the expanded top 50 list, in which expression of both samples in each group was up-regulated or down-regulated two-fold or greater between control SFs and RASFs are presented in Table 4 and in Additional file 4, respectively.
The top 10 up- and down-regulated known isoforms, along with the expanded top 50 list, in which expression of both samples in each group was up-regulated or down-regulated two-fold or greater between control SFs and RASFs are presented in Table 6 and in Additional file 6, respectively.
The top 10 up- and down-regulated novel isoforms, along with the expanded top 50 list, in which expression of both samples in each group was up-regulated or down-regulated two-fold or greater between control SFs and RASFs are presented in Table 8 and in Additional file 8, respectively.
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Quality control samples were included in all sample series, and placed both before and after the patient samples in each analytical run.
(a-g) Mean±SD; n=6 biologically independent samples in each figure panel.
There were n = 4 samples in each group and scale bar = 50 μm.
(a-h) Mean±SD; n=6 biologically independent samples in each figure panel.
(i-k) mean±SD; n=3 biologically independent samples in each figure panel.
There were n = 6 samples in each group and error bars represent SEM.
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