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Because both samples are run in the same gel, gel to gel and other experimental variation is minimized.
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Comparisons between both samples were run to rule out the possibility that differences in posterior factor analyses are due to distinct sample characteristics.
Both the control and the experimental samples are run in a single polyacrylamide gel but imaged separately (Typhoon 9410) at a discrete wavelength for each respective dye.
Both standards and samples were run in duplicate.
Both standards and samples were run in duplicate, and all runs contained an inter-run calibrator to account for any differences between runs.
In accordance with the manufacturer's instructions, all supernatant was collected, stored at −80°C before measurement and both standards and samples were run in triplicate.
Both standards and samples were run in duplicate on a CFX96™ RT-PCR System with a C1000 Thermal Cycler (Bio-Rad, Hercules, CA, USA).
Both quality control samples were run in duplicate before and after each set of samples on each microtiter plate to produce 2 concentration values per quality control sample in each assay.
Samples were run both at the Advanced Light Source (ALS), Lawrence Berkeley National Laboratory, microprobe beam line 10.3 (for Cu wedge and Panoche Creek samples) and NSLS beam line X26A (for Sn wedge samples).
Both the standard curves and samples were run in duplicate.
Both the normal and tumor samples were run on the Affymetrix GeneChip Human Mapping 250 K array for global genomic examination and comparison.
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