Sentence examples for both sample types was from inspiring English sources
Automated extraction for both sample types was performed used the MDX media Kit (Qiagen Ltd, Manchester, UK).
DNA from both sample types was isolated and examined for the presence of mutations in exons 18 21 of the EGFR gene, employing the cobas® EGFR Tissue Test and cobas® EGFR Blood Test (in development, Roche Molecular Systems, Inc., CA, USA).
Quantification curves of both sample types were equivalent, with a detection level as low as 1.6 V. aestuarianus cells in the PCR reaction tube, corresponding to 1.6 · 102 cells ml− 1 and 1.6 · 102 cells mg− 1 in seawater and entire oyster samples, respectively, taking into account the dilution factor used for appropriate template DNA preparation.
Sections from both sample types were cut using a diamond knife (Diatome) at a thickness between 200 and 300 nm.
Both sample types are not essential for early diagnosis of leptospirosis but have value in other situations such as post-mortem investigations.
Both sample types were cultured in trypticase soy broth containing 5 µg/ml gentamicin, on enriched chocolate agar plates, and on selective sheep blood agar plates containing 5 µg/ml gentamicin.
Both sample types were collected on two different occasions approximately 1 month apart.
Within the 56 animals from which both sample types were obtained, CPV-DNA was detected in 46.6% and seropositivity in 64.3%.
Similar amounts of input DNA from both sample types were processed, and microarray data was analyzed using the pipeline described above.
Both sample types were used in this study, as other studies have shown that microRNA levels in matched plasma and serum samples from the same individual are highly correlated (Mitchell et al, 2008; Wang et al, 2012).
