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For both sample types, the abundances of genomes from the Cyanobacteria sibling clade were less than 5% of the total community.> -wrap-foot> 10.7554/eLiFigure02.004 Figure 1.
For both sample types the genes grouped into the same major gene ontology (GO) categories in the same order, with exfoliated cells, having on average 20% fewer genes in each category.
For both sample types, the average was significantly higher in the second evaluation (p = 0.002 for core biopsies and p = 0.02 for surgical samples) but the general shift of level was not significantly different for the two sample types (p = 0.44).
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We observed a high correlation of miRNA expression levels (R = 0.95 and R = 0.98; p < 0.0001) measured for both sample types using the ABI 7900 HT and the 48.48 dynamic array.
Total RNA was extracted from both sample types using the mirVana™ miRNA isolation kit (Ambion) per the manufacturer's protocol.
The tissue-specific abundance patterns of these various subunits were surprisingly complex: the γ-subunit and one β-subunit (#42) were associated with equal spot intensities in both sample types, while the other ATP synthase β-subunit (#44), was 1.8× more abundant in the non-fibre fraction.
By increasing fibrinogen concentration to 4 mg/mL, the zeta-potential threshold attained is almost identical for both sample types, showing that the charge in the erythrocyte surface is partially neutralized by the presence of fibrinogen.
Automated extraction for both sample types was performed used the MDX media Kit (Qiagen Ltd, Manchester, UK).
On day 4, both sample types tested EVD positive, with the venous blood sample showing a higher viral load compared with the capillary blood sample.
DNA from both sample types was isolated and examined for the presence of mutations in exons 18 21 of the EGFR gene, employing the cobas® EGFR Tissue Test and cobas® EGFR Blood Test (in development, Roche Molecular Systems, Inc., CA, USA).
Again, the Ct values from both sample types were highly correlated.
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