For sample illumination and focusing, white light is coupled via an optical fiber into the set-up and applied in transmission.
This chapter presents an overview of the complex and ever-developing landscape of imaging cytometry, highlighting the imaging and quantitative performance of a wide range of available instruments based on their methods of sample illumination and the detection technologies they employ.
The XRF spectra were recorded with an ARTAX-200 μ-XRF spectrometer (Bruker), equipped with a molybdenum X-ray tube, an integrated CCD camera with sample illumination and laser spot, a silicon drift detector with a resolution of <150 eV and a 0.65 mm collimator.
The 561-nm laser lines were used for sample illumination and a 63×/1.4 n.a.a
A quad-band dichroic and emission filter set (LF405/488/561/635-A; Semrock) set was used for sample illumination and emission.
The second approach, known as FTIR imaging, does not use an aperture, but incorporates wide-field sample illumination and detection using a focal plane array detector to quickly acquire FTIR images of substantial areas of the sample.
The housing is made from an acetal copolymer, which we have found causes minimal background fluorescence, and a manifold in front of the objective admits X-rays, contains a sample illumination and web-cam-based viewing system, and allows other detectors, such as the Vortex, a view of the sample.
The body of the microscope consists of three main parts: (i) A light tight sample housing with a varied selection of stubs and mountings and ports at 10°, 45° and 60° for admitting x-rays, sample illumination systems, sample viewing cameras and supplementary detectors.
In our case, without SOA2, the measured sensitivity was 81 dB, which was smaller than the calculated shot-noise-limited sensitivity of 107 dB by 26 dB, for a 2.5-MHz A-scan rate and a sample illumination power of 20 mW.
As seen from Figs. 3 and 4, the kinetics of illumination and darkening of RC solution is depended on the intensity and time of sample illumination.
