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Creative solutions to improve or exploit the photophysical drawbacks of fluorescence imaging have been presented in both sample and instrument forms.
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Details of both the sample processing and instrument settings and handling have been described elsewhere [ 70].
Hence, the sample quality and instrument resolution are critical.
MS prepared documents for sample preparation and instrument analysis.
H6-25(OH)D3 was used as internal standard (IS) to correct for sample treatment and instrument variability.
Both sample analysis instruments were able to "sniff" the gases present near the surface.
Barcoding will note reagents, samples, and instruments used, providing context to the data for subsequent analysis.
This data collection will involve independent samples and instruments in order to answer each evaluation aim.
This behavior can be attributed to heat transfer between the sample and the instrument.
Internal quality control samples were run every 10th sample, and the instrument was recalibrated every 15th sample.
The sample and setting, instrument, data collection, and data analysis are detailed in the next section.
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