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The DNA from both sample and input was amplified by semi-quantitative PCR (30 cycles) using the primers specific for the CCL11 promoter (–136 to +61) encompassing both STAT and NF-κB binding sites.
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Unlike the traditional identification, the PE conditions under irregular and random sampling involve both sampling times and input values.
The primary objective of Expedition 334 was to both sample and quantify the sediment inputs to the seismogenic zone of an erosive subduction margin.
Purified sample and input DNA was eluted with 50 µl EB buffer.
Chromatin precipitated DNA was resuspended in a final volume of 40 ul of water (both for IP samples and input samples) and 1/10th of each was used for the PCR amplification.
As shown in Figure 7A, after Pax8 chromatin immunoprecipitation followed by semiquantitative RT-PCR, we observed an enrichment of IP regions for all the above-mentioned validation targets in comparison with both non-IP samples and input samples.
JDR provided control samples and input on the manuscript.
The mean of 6 assays (variation 0.6 log) was taken as the input RNA amount of the sample, and sample input RNA was normalised accordingly.
Similarly, there is a 100% concordance by risk classification across all samples and inputs.
It contains one FoxA1 ChIP sample and one input control sample.
For each individual, both ChIP and input samples were sequenced.
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