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Tris-HCL buffer (pH 8.9) and 1.5 mM DTNB were added to 1 mL supernatant for both sample and control.
The probes/genes for these analyses were filtered by requiring the Lowess normalized intensity values in both sample and control to be >10.
The probes or genes for all analyses were filtered by requiring the lowest normalized intensity values in both sample and control to be > 10.
The probes or genes of the combined UNC337 and UNC105 data set for all analyses were filtered by requiring the lowess normalized intensity values in both sample and control to be >10.
Next, after normalizing both sample and control libraries to the same size (one million reads), the significance of enrichment is determined for each of the "eligible" windows or domains (a cluster of contiguous windows) relative to the control library.
Prior to analyses, the expression data were downloaded from the UNCMD, and the probes were filtered by requiring the Lowess normalized intensity values in both sample and control to be greater than 10 dpi and present on more than 70%% of microarrays.
Similar(54)
The reaction was terminated by adding 200 µl of 30% acetic acid and absorbance measured at 410 nm in both samples and controls.
After centrifuging at 10,000 g for 10 min, an equal volume of 1M NaOH was added to the supernatant and absorbance was measured at 450 nm in both samples and controls.
The probes/genes were filtered by requiring the lowest normalized intensity values to be greater than 10 in both samples and controls.
Plasmids were linearized with restriction enzyme NdeI (New England BioLabs) before use and mixed with DNA from Daudi cell line in order to run the amplification reaction under identical mass/volume DNA concentration for both samples and controls.
In this method, the sample including the target simultaneously plays the role of both sample and negative control.
More suggestions(15)
both freedom and control
both service and control
both expense and control
both study and control
both operation and control
both promotion and control
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both sample and input
both sample and tip
both treatment and control
both conservation and control
both measurement and control
both sample and calibration
both ownership and control
both analysis and control
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