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In addition, a polypyrimidine tract-binding protein (PTB -encoding gene (LOC_Os03g25980) was uPTB -encodingn both rice lines at low temperature.
Four genes encoding ribosomal proteins (LOC_Os08g03450, LOC_Os01g64090, LOC_Os01g69950 and LOC_Os07g12250, encoding L37, L1p/L10e, L27 and L24, respectively) were up-regulated in both rice lines at low temperature.
Our results show that RSZpZ23 was down-regulated in both rice lines at low temperature, but RSZpZ21a was down-regulated in Co27LT.
The 40S subunit first binds eIF3 and an eIF2-GTP-initiator transfer RNA ternary complex, then the 48S initiation complex is assembled with the help of many eIFs via numerous steps [ 83]. eIF5B (LOC_Os05g51500), which was most strongly expressed in both rice lines at low temperature, is essential for the binding of 60S subunits and dissociation of eIF1, eIF1A, eIF3 and residual eIF2 GDP [ 84].
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The seeds of both rice lines were germinated and grown in petri dishes for 6 d in a growth cabinet (28°C during the day and 24°C during the night, 16/8 h photoperiod at 50 μmol m 2 s–1).
The hypoxia-related genes SUS1 and ADH1 were expressed more strongly under submergence in both rice lines.
Activity of SOD, the enzyme responsible for converting highly active O2 − was substantial in both rice lines and was little changed by submersion.
Our quantitative RT PCR results revealed that SUS1 expression was increased by submergence in both rice lines, with a proportionately much larger increase in 'FR13A'FR13A
Submergence promoted chlorosis in both rice lines although older leaves lost more chlorophyll in 'FR13A' and younger leaves lost more in 'TL3'TL3
Set 6 type of expression occurred in all the three groups of genes in both rice lines Rb49 and IRBB13 (Figure 2).
This type of expression also occurred in all the three groups of genes in both rice lines Rb49 and IRBB13 (Figure 2).
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