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We also observed significant enrichment for diet-specific responses, with 692 SNPs showing significant deviation in allele frequencies between both replicates of one diet and baseline (at P < 10−5) but not in the other diets.
The main concern rises from the comparison with previously described studies as shown in Figure 6 figure supplement 2. The authors state: "Furthermore, and consistent with our observations, REX1 is either absent or expressed at low levels in both replicates of one of two retrovirally reprogrammed bonobo (Pan paniscus, sister species to chimpanzees) iPSC lines generated by (Marchetto et al. 2013b)".
A total of 360 genes were identified as differentially methylated between the two cell lines (i.e. methylated in both replicates of one cell line, but not in both replicates of the other) and did not have MYCN binding.
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Figures represent means and SD of replicates of one representative experiment each.
Genes that were expressed in both replicates of at least one sample at 5 or more normalized TGR counts were selected for further analyses.
We conservatively focused on the eight outliers that showed parallel divergence in both size-selected replicates of one selection line.
The experimental design was completely randomized with six treatments and five replicates of one quail.
with Var being the variance between the replicates of one sample, and n the number of samples.
Data are presented with the mean ± standard deviation (for six replicates of one chick per cage).
Four Petri dishes contained 8 biological replicates of one floret organ and represented one independent experiment.
Three field replicates of one half square meters each were hand harvested leaving two inches stubble.
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