INH is known to specifically kill multiplying bacteria and is not bactericidal against non-replicating dormant bacilli [4], [21], [46], while RIF kills both replicating and non-replicating mycobacteria.
We compared inhibitors of both replicating and non-replicating phase, and observed that molecular weight, hydrogen bond acceptor, atom count, polar surface area, and rotatable bond count is significantly lower (p < 0.05) in the compounds inhibiting replication phase of M.tb.
All activity of OPB was shown to be dependent on the acid- and NO dependent conversion of OPB to 4-OH-OPB, which was active on both replicating and non-replicating Mtb.
These data indicate that mono-indolizine mono-salt 6d is a potent compound against both replicating and non-replicating Mycobacterium tuberculosis, is active against both extracellular and intracellular organisms, has a bacteriostatic mechanism of action and has basically no toxicity.
Screening of the compound libraries designed for bacterial phosphotransferases resulted in the discovery of a selective WecA inhibitor, UT-01320 (12) that kills both replicating and non-replicating Mtb at low concentration.
Inhibition of WecA will block the entire biosynthesis of essential cell wall components of Mtb in both replicating and non-replicating states, making this enzyme a target for development of novel drugs.
To examine the properties of 2G12 transiently expressed in N. benthamiana using both replicating and non-replicating CPMV vectors, two series of plasmids were used.
There have been several previous reports of the successful production of full-size antibodies in plants using both replicating and non-replicating transient expression systems.
The mechanisms by which CGIs are protected from methylation in both replicating and non-replicating chromatin in normal cells, and the mechanism(s) whereby these DNA regions become susceptible to methylation in tumor cells are still unknown [2], [8], [9].
The work reported in this manuscript describes an extensive characterisation of a purified full-length IgG produced transiently in plants using both replicating and non-replicating CPMV-based systems.
To assess the quality of antibodies transiently expressed to high levels in plants, we have expressed and characterised the human anti-HIV monoclonal antibody, 2G12, using both replicating and non-replicating systems based on deleted versions of Cowpea mosaic virus (CPMV) RNA-2.
