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Initial genome architecture was examined by using both repetitive element analysis and genome synteny.
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Repetitive element analysis was performed by using the RepeatModeler and RepeatMasker programs (http://www.repeatmasker.org).org
Repetitive element analysis was conducted as described above using RepEnrich software.
Repetitive element analysis revealed numerous LINE-L1 elements at regions where conservation is lost among the Sry copies.
To test the repetitive element analysis we simulated ChIP-seq data on human chromosomes 5, 10, and 19 (see Additional file 1: Figure S2 for HMM parameters).
Previously, the repetitive element content of the M. oryzae genome was described (Dean et al. 2005; Xue et al. 2012); however, repetitive element analysis had not been performed on M. poae or G. graminis var.
For repetitive element analysis, we made a custom dataset using all SINEs/LINEs/EhEREs coordinates recently deposited to AmoebaDB (personal communication, Omar Harb), then aligned all sequences with Bowtie using the parameters -v 2, -k 5.
To examine how repetitive element methylation was related to gene-associated methylation, we restricted our analysis to placentas for which we had both repetitive element and gene-associated (array) methylation data for the 26,486 CpG autosomal loci (n = 184).
It proved to be a robust method for imaging of both repetitive elements and coding genes.
These results suggest that lower global DNA methylation, assessed by LINE-1 repetitive elements methylation analysis, would be associated with a greater risk for MetS in the presence of obesity.
Only spots with annotated DNA sequence with 90% or more of their length overlapping a repetitive element were used for analysis.
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