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The availability of marmoset transcriptome data from five tissues, including both raw sequences and assembled transcripts, will aid in the annotation of the newly released marmoset assembly.
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Values are shown for both raw sequence and the sequence after trimming from reads with low quality bases.
Briefly, primer and vector sequences were removed from raw sequences and quality of sequence determined.
Raw sequence and mapped data for this study has been deposited in the Gene Expression Omnibus (GSE30765).
Following previous barcoding studies, the number of variable sites within species, maximum intraspecific uncorrected p-distance, and minimum interspecific uncorrected p-distance were calculated for the six species that are polymorphic for the inversion region, using alignments based on both the raw sequence matrix and the uniform inversion matrix.
Both the raw sequencing reads and assembled sequences have been submitted to NCBI, which has been supplemented by a pennycress genome database containing a JBrowse genome browser, BLAST database, and data repository available at pennycress.umn.edu.edu
Raw sequences were demultiplexed and then quality-filtered using the default parameters in QIIME.
Raw sequences of metagenome and metavirome from BBMO sample have been deposited in the European Nucleotide Archive under the accession number PRJEB12379.
Raw sequences were cleaned and assembled into contigs.
Raw sequences were edited and assembled with GENEIOUS v.4.6.5 [ 39].
The massive amount of sequencing reads obtained from L. menadoensis liver and testis allowed us to apply stringent filtering criteria, both in the processing of raw sequencing reads and in the filtering of assembled contigs, in order to achieve a final set of high quality transcripts and to overcome the most common pitfalls of NGS assemblies.
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