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No statistically significant differences were found between the two EGFP-Ptetbi-luc lines used, confirming that both rat lines show equal efficacy in reporting Ptet-controlled gene activation.
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A recent study using selected rat lines showed a genotype-dependent impact of maternal separation on alcohol intake in rats [33].
Mild concordance with the human literature has been achieved for the cortisol response to alcohol, with a high-drinking selected rat line showing decreased corticosterone (the rodent analogue of cortisol) response to alcohol relative to the low-drinking line (Apter and Eriksson 2006).
Small variations were indeed seen in the expression of the traditional housekeeper genes (see Table 2), while the 4 selected invariant genes showed consistent expression across both rat lines and in both cohorts (see Figure 2 for examples).
On day 8 both drugs in both cell lines show tRNA synthetase downregulation.
Both cell lines show human karyology.
In addition, both cell lines show elevated SHP2 expression [ 28].
In the extinction trial (day 2), no freezing behaviour was displayed during the first 5 min in the extinction chamber (prior CS presentation, trial 0), indicating excellent discrimination of context B and A. Rats of both lines showed similarly pronounced expression of fear (around 80% freezing) during the first CS presentation.
Studies using both Fisher 344 and Sprague-Dawley (SD) rat lines have shown that gestational and/or lactational maternal lead (Pb) exposure causes delayed reproductive maturation in their respective female offspring.
The thick lines show the means across rats.
Both cell lines showed similar responses.
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