There are two main types of mutagenesis, i.e., random and site-directed mutagenesis.
Yeast display libraries of random and site-directed hVβ2.1 mutants were selected for improved domain stability and for higher affinity binding to TSST-1.
We have used random and site-directed mutagenesis to identify residues in VirS that are important for its function and have identified a region in the putative sensory domain of VirS that appeared to be essential for function.
In addition, several recent genome-scale random and site-directed mutagenesis studies have led to the identification of novel genes important for replication inside macrophages and/or survival in mice [7], [19], [20], [21], [22], [23], [24].
A CotA double mutant, K316N/D500G, was constructed by combining random and site-directed mutagenesis.
In summary, the combination of random and site-directed mutagenesis resulted in the B. licheniformis CotA double mutant K316N/D500G.
Herein, random and site-directed mutagenesis methods were combined in order to improve the functional expression of CotA in E. coli.
In this study, random mutagenesis and site-directed saturation mutagenesis were used to enhance the hydroxylation activity of H6H from Anisodus acutangulus (AaH6H).
By random mutagenesis and site directed evolution, it has been possible to expand the substrate specificity, as well as to improve and change the stereoselectivity of cyclopentanone monooxygenase (CPMO) (Clouthier and Kayser 2006) and CHMO (Zhang 2013), among others.
In both random and directed biopsy, there was a statistically significant pattern of CIN occurrence on the cervix (χKWd = 54.3880, χKWr = 73.1819, p < 0.0001).
