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Some labs have reported that at least one of the RS domains of SF2/ASF and U1-70K is required for interactions between these two proteins [13], [67], but these experiments did not demonstrate that the RS domains of both proteins were required for their interaction.
Bendezu et al (2009) constructed a fully functional mreB∷mCherry sandwich fusion and obtained convincing evidence that the formation of MreB and RodZ helices were interdependent, that is, both proteins were required for the helical structures to form.
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Both proteins are required for proper sister chromatid cohesion, but their exact function is unclear at present.
Whether the effects of YC137 are driven by inhibition of BCL-W, BCL2, or inhibition of both proteins is required, is unknown.
Genetic perturbation of either Yox1 or Nrm1 leads to increased MBF-dependent transcription indicating that both proteins are required, but are not sufficient, to repress MBF transcription outside of G1 phase.
Quantification showed that GH-stimulated GHR endocytosis is reduced from 50% to about 20% under conditions of either clathrin or βTrCP depletion, indicating that both proteins are required for GH-induced GHR endocytosis.
Both proteins are required for the autophagosome formation [ 91].
Both proteins are required to target Dnm1 and are essential for efficient nucleation.
Alternatively, both proteins are required to assemble and/or stabilize a piRNA cluster regulatory complex.
This suggests that both proteins are required for pathway function in all Pezizomycotina in which they occur.
Similarly, both proteins are required to establish Tg(7xTCF-Xla.Siam:GFP) 7xTCF-Xla.Siam GFPforming DRGs at 54 hpf.
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CEO of Professional Science Editing for Scientists @ prosciediting.com