Therefore, the authors should provide some control ITC traces looking at buffer injections into the protein, ligand injection into buffer (no protein) and buffer to buffer.
The buffer capacity of human blood is relatively high due to the presence of proteins and buffer salts.
Protein concentrations and buffer compositions are mentioned in the text.
The UPR is activated upon the accumulation of misfolded proteins and buffers the ER stress.
In some buffers, the interaction between the protein and the buffer constituents is so slow that the peaks become broad.
Both DR1 and DM proteins were purified and buffer exchanged into PBS (7 mM Na+/K+ phosphate, 135 mM NaCl, pH 7.4) using centrifugal ultra-filtration (Amicon).
The column was then washed with 5 ml cold GST binding buffer and protein extraction buffer, respectively, and 2 5 ml of total root protein in cold protein extraction buffer were loaded.
Both phosphate and sulfate were present in the protein storage buffer and in the crystallization conditions, respectively.
The pellets were eluted in protein loading buffer, and samples were vortexed and boiled for 5 mins at 95°C.
Examples of these are purity and aggregation state of the protein, buffers and their pH values, temperature, precipitants and additives.
