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The key target cells for the virus in the lung are the alveolar epithelium and alveolar macrophages, and we have shown that, compared to seasonal human influenza viruses, equivalent infecting doses of H5N1 viruses markedly up-regulate pro-inflammatory cytokines in both primary cell types in vitro.
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That is, despite both neurological and non-neurological Ndufs4 fky/fky primary cell types manifesting reduced CI activity and disrupted CI formation, the effect on mitochondrial function differs.
The miniChIP chip approaches described here can be utilized to identify the chromatin states of primary cell types that are present as both rare and abundant populations in tissues and organs across many model organisms.
The primary cell types of tendons are the spindle-shaped tenocytes (fibrocytes) and tenoblasts (fibroblasts).
Two primary cell types make up the pineal gland.
We demonstrated its utility in several cultured and primary cell types.
Whereas viral strategies are time consuming and involve safety risks, non-viral methods proved to be inefficient for most primary cell types.
Macrophages in particular are believed to be among the "first responders" and primary cell types that uptake and process nanoparticles, mediating host biological responses by subsequent interactions with inflammatory signaling pathways and immune cells.
Using commercial magnetic beads, we achieved efficient purification of HIV-1-infected cells (i.e. purity > 85% and recovery > 90%) from diverse primary cell types at early time points following infection.
These effects involve the release of specific CKs and GFs within the local environment [7], resulting in recruitment of two primary cell types to the site, inflammatory cells and progenitor cells (MSCs and HSCs) [19], which in turn regulate the subsequent healing processes.
The goal of this study was to determine the elastic and viscoelastic properties of three primary cell types of mesenchymal lineage (chondrocytes, osteoblasts, and adipocytes) and to test the hypothesis that primary differentiated cells exhibit distinct mechanical properties compared to adult stem cells (adipose-derived or bone marrow-derived mesenchymal stem cells).
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