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The changes in allele frequencies between pre and post-epidemic samples in both populations were then computed using an exact homogeneity test implemented in GENEPOP v3.4.
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Both cell populations were then mixed after lysis, and co-immunoprecipitations identified interactions between the two molecules that could not have happened in the cells 51.
The MGCs retained in the 30-μm sieve were flushed out with alpha-MEM medium containing 10% v/v FCS, and both cell populations were then resuspended in complete alpha-MEM containing IL-4 and cultured overnight (37°C; humidified; 5% CO2).
CFSE-labelled primary NK cells and both CD56bright and CD56dim populations were then cultured for up to 7 days in the presence of IL-2 or IL-15 with or without the addition of IL-21.
These cell populations were then evaluated for their osteogenic, adipogenic, and chondrogenic differentiation capabilities.
Cell populations were then cultured without puromycin.
Positive parasite populations were then cloned by limiting dilution.
Populations were then propagated in a static incubator at 28°C for 3 days.
These populations were then subjected to drug cycling protocols to enrich for integrants.
The two populations were then recounted and remixed within an electroporation cuvette at 50% cell ratio for exposure.
Populations were then switched to 2i/LIF/N2B27.
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