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Fluorescent-activated cell sorting analysis revealed that both populations were negative for CD14, CD34 and CD45 expression and that both displayed equal levels of CD44, CD73, CD90 and CD105, a phenotype currently known to be characteristic of BM-MSC.
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Furthermore, the parental HUVEC populations were negative for CD45 and CD34, ruling out the contribution of any possible residual hematopoietic cells obtained from HUVEC preparations in the high reprogramming efficiencies observed (Figure 4A).
In addition, 2,276 serum samples from the same populations were negative for influenza-specific antibodies.
These fibroblast populations were negative for cytokeratin, whereas they expressed fibroblastic marker vimentin, which suggests that some fibroblast traits were preserved in the CAFs.
The Fs values of the entire P. vivax populations as well as the East Asian and Myanmar populations were negative and significant (P = 0.025 for East Asian populations and P = 0.005 for Myanmar population).
Also, the chronic equilibrium does not exist because the infected T-cell and free virus populations are negative.
We also noted that all D, D* and F* values across populations are negative for fester.
A review of risk factors for reinfection, such as travel to areas endemic for TB or exposure to high-risk populations, was negative, suggesting that the second strain was acquired before treatment.
Most of the cells within the CD31+CD45− population were negative for 7AAD staining, indicating that these cell were viable.
According to qualifications, the percent changes in nurses per population were negative in three SMAs: Tankou (−0.8%), Ryouban (−4.0%) and Sousou (−33.7%).
The percent changes in total nursing staff per population were negative in four SMAs: Ryouban (−4.0%), Ishinomaki (−1.9%), Sousou (−47.7%) and Iwaki (−1.9%).
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