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Both populations were allowed to adhere, proliferate and recover for 3 days prior to retroviral transduction.
Both populations were allowed to differentiate for one week after sorting and assayed for SMA, nestin, MAP2 and Ki67 markers.
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But that was not what happened.Both populations were allowed ten days to evolve.
Adults from both coevolved and control populations were allowed to deposit eggs in parasite-free medium.
A merchant mariner said Davao's Muslim, Christian and indigenous populations were allowed to "deal with their own problems, including criminals" to prevent sectarian anger from outside interference.
Populations were allowed to evolve for 30 days (c. 200 generations), with daily transfer to fresh medium.
Post bottleneck populations were allowed to grow exponentially at 10, 20 and 50%% for 34 100 years.
Paclitaxel-surviving Populations were allowed to grow for 2 3 weeks in paclitaxel-free culture medium.
Initially clonal populations were allowed to evolve under conditions where selection late in life was very weak.
Brachionus populations were allowed to grow for 12 days (with periodic medium replacement) at which time sampling was initiated.
Nematode populations were allowed to expand until food sources were exhausted, after which the worms were harvested for DNA extraction, using previously-established protocols for C. elegans [ 43].
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CEO of Professional Science Editing for Scientists @ prosciediting.com