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To address this, 10 of these transcripts that showed inconsistent results from RNA-Seq and DGE platforms were randomly selected to assess their relative expression patterns among CK, CA1 and CA3 using quantitative RT-PCR approach (qRT-PCT).
Compared with array-based approaches that only enable one to examine genomic regions covered by predefined probes, short reads from NGS platforms are randomly sampled from the entire genome and provide single nucleotide resolution [ 30].
The platform was randomly placed in one quadrant for the duration of the experimental procedure.
For pre-training, the platform was randomly positioned either 20 cm or 40 cm from the edge of the pool, each for two trials per session.
The platform was randomly positioned either 25 cm or 50 cm from the edge of the pool, each for two trials per session.
Details regarding ACE assay robustness and reproducibility are described in Additional file 1. Preserving read pair information, the original amount of sequence data collected for each WES platform was randomly downsampled to control either the total amount of sequence data in Gigabases (Gb) or the mean depth of coverage in each platform's target regions.
Only probe sets present in both platforms were processed.
Both platforms were manufactured by NimbleGen Systems (http://www.nimblegen.com).
The efficiencies obtained on both platforms are similar.
Both platforms are elevated and each has a wheelchair ramp.
At the basis, both platforms are complementary in many regards.
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CEO of Professional Science Editing for Scientists @ prosciediting.com