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After incubation (aerobically, 48 h at 37 ± 2°C) the number of CFU was counted, the mean number of CFU in both plates was calculated and transformed to a log10-value.
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The resulting mutant strain (Δ SOP) was cultivated on a solid medium, and the number of spores collected from the plates was calculated from 5 to 14 days, and the yield of Gdm was measured by high-performance liquid chromatography.
The wear depth and wear track profiles after the friction tests were obtained by a noncontact 3D surface profiler (Olympus OLS4000), and the wear volume of the plates was calculated from the wear depth.
Mean clear zone of these plates was calculated with standard error.
The number of 150 mm plates was calculated based on the library titer.
At each time point, the fraction of the population that contained the plasmid on the original YPD plates was calculated from the selective plates as CFUgrowth/CFUtotal.
The number of theoretical plates was calculated using our in-house MATLAB-based program (P/ACE 2100) or 32 Karat software (32 Karat™ Version 8.0 Workstation, Beckman).
Both the intra-variation within one plate and the inter-variation between plates were calculated.
Frequencies at the tip of the fluttering plates were calculated using a Fast Fourier Transfer FFTT) at varying temperatures.
The global buckling strength of welded stiffened plates is calculated according to Mikami and Niwa.
Based on electropherograms, the number of theoretical separation plates is calculated (max. 70,000) and compared with data from the literature.
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CEO of Professional Science Editing for Scientists @ prosciediting.com