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After 2 h incubation at 37°C, cells containing both plasmids were selected by using a 96-prong frogger to transfer cells to LB plates containing ampicillin and tetracycline.
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ADE+ GT17 cells harbouring episomal URA3 plasmids for the production of Sup35Q54 or Sup35Q92 were plated onto 5-FOA selective medium, and cells lacking the expression plasmids were selected [41].
These two plasmids were introduced into E. coli codonPlus™ (DE3 -RIL cells (Stratagene, La Jolla, CA, USA) forcibly against incompatibility, anDE3 -RILlls cellsining the two plaStratagene seLa Jolla, CAe agar plate containing both kanamycin and ampicillin.
In the second step, plasmids were selected for their ability to induce protective immunity in a mouse model of tuberculosis following DNA immunization.
Yeast clones harboring the bait plasmids were selected on SD/-Trp medium.
Plasmids were selected using ampicillin (100 µg/ml) with chloramphenicol (15 µg/ml).
Cells transfected with pN series plasmids were selected with G418 as described [47].
Six sequenced Campylobacter plasmids were selected for the comparative analysis (the supplementary Table S2).
Yeast carrying "bait" and RNA expression plasmids were selected on –Trp, -Ura plates and screened as above.
Cells transfected with shRNA expression plasmids were selected in the presence of zeocin (250 µg/ml) [45].
E. coli and GBS clones carrying the pG+host5 and pP1 plasmids were selected in the presence of 300 µg/ml and 2 µg/ml erythromycin, respectively.
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