Our paradigm of stereotactic injection of AAV into the ARC or PVN does not permit correlations between physiological end points and levels of gene expression, because only a subset of the total neurons within each nuclei were infected.
The results of the physiological end points for the individual studies are summarized in Figures 2 and 3. We separated studies with an active control group (for example, hyperinflation by the ventilator) from studies comparing MH with standard care.
For example, toxicologists have identified a need to understand the risks posed by compounds that do not exhibit a monotonic dose response curve or affect different physiological end points differently in various concentration ranges (Talsness et al. 2009).
Phase 3 studies conducted to examine efficacy of diabetes agents rely, by design, on short-term assessment of physiological end points, such as A1C levels, which are considered predictive of improved medical outcomes when followed over time.
However, it is a "double-edged-sword" as both over-, and under-filling can be harmful, therefore it should be performed according to adequate physiological end-points.
Thus, the physiological end-points in our experiments; liver size and xenobiotic metabolism, were tested in Conv-D mice 1 week, 3 weeks and 6 weeks after conventionalization.
One of the primary physiological end-points of these upstream signal regulation processes is reduced neutrophil infiltration into tissue [4], [29], but how the 'molecular' processes affected by CO link to this endpoint is unknown.
Furthermore, there were no controlled studies of thoracentesis for any clinical or physiological end-point.
We compared and critiqued a number of physiological end-points (Table 2).
The authors of that review also assessed the use of physiological end-points such as tissue perfusion and oncotic pressure.
