Sentence examples for both phosphorylated and unphosphorylated from inspiring English sources

In order to validate the phosphorylation-states of AMPK-α both phosphorylated and unphosphorylated AMPK-α were detected in lung homogenates from S. pneumoniae infected animals (Figure 4b).

We could clearly show that YopM interacts with both phosphorylated and unphosphorylated RSK1 (Fig. 5C).

Western blots were then probed with an antibody that recognizes all forms of RNAP II, both phosphorylated and unphosphorylated.

As shown in Fig. 2a, phosphorylation of AMPK at the Thr172 residue on the alpha-subunit was drastically increased in co-culture compared with MDCK alone whereas total AMPK remained unchanged as shown in a blot probed with AMPK (pan-α) antibody that recognizes both phosphorylated and unphosphorylated AMPK.

We first confirmed that both phosphorylated and unphosphorylated Akt proteins were considerably decreased in cultured Akt1-/ osteoblasts without compensatory increase of Akt2 protein, indicating a substantial suppression of the total Akt signaling by depletion of an isoform Akt1 in osteoblasts (Fig 2A).

Both phosphorylated and unphosphorylated MYPT are downstream targets of ROCK.

In other cases, both the phosphorylated and unphosphorylated forms of the protein participate in the same PPI, indicating that phosphorylation is dispensable for the interaction.

Early mutational studies of the DegS/U two component system established that the response regulator binds DNA sequences and regulates expression of specific genes both in its phosphorylated and unphosphorylated state.

Unphosphorylated Hsp25 is preferentially localized in the perinuclear region of the cytoplasm, whereas both the phosphorylated and unphosphorylated proteins are present at the lamellipodia after stimulation [33].

Alternatively, the binding of Nkd1, but not Nkd1G2A, to Dvl, may lead to Dvl phosphorylation, but this does not account for the observation that Nkd1 interacts with both a phosphorylated and unphosphorylated form of Dvl.

Based on the observation that PTPMeg2 interacts with STAT3 in the absence of IL-6, we concluded that PTPMeg2 interacts with both the phosphorylated and unphosphorylated STAT3.