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In this chapter we will discuss the formation and differentiation of CD4+ T lymphocyte subsets, including the central memory subsets based on both phenotype and function.
Age-associated changes in both phenotype and function have been reported for many cell types, including T cells, B cells, natural killer (NK) cells, and follicular dendritic cells (FDCs; for review see [ 3]).
In contrast, effective removal of HCV by an IFN-free regimen of direct-acting antivirals normalizes both phenotype and function of NK cells, as now shown in two independent studies on combination therapy with the HCV NS5A inhibitor daclatasvir and the NS3 protease inhibitor asu-naprevir.
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Moreover, there is conflicting information about both the phenotype and function of these cells [1], [4], [5], [6], [7].
Therefore, it is important to consider both the phenotype and function of vaccine-induced T cells when evaluating their protective potential.
FCM can thus measure both the phenotype and function of specific cell subtypes in a sample that may contain many different cell subtypes, unlike assays like microarrays and proteomics that measure aggregate features of the entire mixture of cells.
Adiponectin has been shown to modulate both neutrophil phenotype and functions, but its significance in the PH development remains unknown.
Nonetheless, ECs from both atrium and ventricle maintain their phenotype and function in vitro.
There is an ongoing debate about an association between phenotype and function, as both granulocytic and monocytic MDSCs have been reported to exhibit immune suppression in a disease- and tissue site-dependent manner [ 12, 28].
The role of hypoxia on subverting macrophages to TAM/M2 phenotype and function likely involves both HIF-1α-dependent and independent processes.
These engineered cells retained antiviral specificity and functionality, and contained a subset with regulatory phenotype and function.
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