Lula/Lambda double lysogens can be easily generated (Fig. 2B), indicating that the two phages are different not only in immunities, but also in attachment sites.
Phage display and other high-throughput methods facilitate selection of internalizable antibodies and their fragments (keeping in mind that multivalent interaction of the ligand-exposing phages is different from that of ligand).
Then, I suppose, the argument would be that the trees of the bacteria and phages are different, suggesting that there has been some exchange of prophages and individual genes.
4) I am not so convinced by the argument on page 3 that the results imply that even among closely related prokaryotes the most commonly encountered phages are different.
Based upon the biological differences (including host range and plaque formation differences), the significant genetic alterations (including the presence of "indels"), and the protein level differences (highly variable but related protein sequences) presented, we conclude that each of these phages are different but related phages.
If the PFGE profiles were related, but the phage types were different, ribotyping with one to three restriction enzymes (HindIII, EcoRI, ClaI ) was performed to verify the relatedness of isolates (11 ).
The results for Hyperphage packaged phage (Fig. 4b) were different.
However, the reactions to the typing phages in RDNC-a strains were different from those in PT6a.
There is evidence for exchange of large blocks of genes that produce fully functional phages that are different from the inferred parents [ 8, 9].
When Seed et al. looked at a phage-positive stool sample from a Bangladeshi patient, more mutations that made the bacteria resistant to this phage were found; however, these mutations were different again from the ones in the Haitian bacteria.
