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From the size and sequence of the products (Figure 2d f), and our Northern data, we conclude that there are two principal Math5 transcripts in the retina, 1.7 kb and 4.4 kb in length, and that both of these transcripts are unspliced.
Specifically, we observed a trend for high- grade ER+ (i.e. Luminal B) tumors to express high levels of both of these transcripts (data not shown); therefore, PTK6 may contribute to the growth and survival of these tumors by modulating IGF-1R signaling.
We found that both of these transcripts produced shorter processing products that were uniquely present in exosomes.
If they map to different transcripts, they are classified as unannotated splice variants if both of these transcripts belong to the same gene.
The fact that both of these transcripts were detectable, even at greatly reduced levels, indicates that RLSB expression was not completely silenced.
TransDecoder predicted a coding region in one of these transcripts, and given their high GC content and sequence similarity, we reasoned that both of these transcripts likely represented unannotated pseudogenes.
Similar(47)
Despite similar fold changes in expression for both mutants, many of these transcripts were called differentially expressed in only one mutant due to our adjusted p-value filtering.
We thus confirmed the expression and sequence limits of both these transcripts in the embryonic mouse brain using both RT PCR and 5′/3′-RACE (KF032589 and KF032590).
Both of these longer transcripts could theoretically interact with the full-length MicA RNA although the third band would only overlap by about one helical turn (14nts in this study, 15 nts in [23]) precluding RNase III processing; RNA duplexes formed are targets for RNase III [17] which specifically recognizes and cleaves double-stranded RNA of at least two helical turns [36].
Both of these chitin_bind_4 transcripts were up-regulated in intermoult when compared to early pre-moult.
We independently validated changes in the steady state levels of 30/44 (68%) of these transcripts from both lists by qRT-PCR (Fig. 6B; Table 1).
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