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Both of the constructs mentioned in Theorem 18 give a systematic approach to what had been previously longstanding ring-theoretic questions.
To achieve transient expression of YFP-paxillin and GFP-actin Swiss 3T3 and REF-52 cells were transfected with either one or both of the constructs by nuclear microinjection.
Both of the constructs were introduced into the Japonica rice Zhonghua11 (ZH11) via the A. tumefaciens-mediated transformation method.
After both of the constructs were confirmed by sequencing, the plasmids expressing N-terminal His6-tagged BlaC WT and mutant proteins were transformed into Escherichia coli BL21/DE3 cells and cultured in LB broth at 37 °C.
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When mirroring the findings with the literature, both the emergences of the constructs were compared and unexpected types were considered.
To address this issue, in this study we use specifically developed statistically separable measures of I, A and P. We used structural equation modelling (SEM) as these methods can evaluate models of both the measurement of the constructs and the structure of the relationships between constructs.
Addition of streptavidin to the cis compartment strongly hindered occlusion by both of the biotinylated constructs but had no effect on occlusion by DTA constructs lacking the biotin label.
HEK-293T cells transfected with wild-type and tumor-derived FAS pKEX constructs were assayed for exogenous FAS expression, in order to see both the proper functionality of the constructs and the possible variations in the expression levels among samples.
Both of the C/EBPα constructs significantly (p < 0.01) inhibited the PSA promoter although the pcDNA3-C/EBPα construct inhibited PSA transcriptional activity more than the C/EBPα-pEGFP construct both under basal conditions and in the presence of DHT.
Both of these constructs failed to reproduce the proliferative effect of the complete fusion, demonstrating the importance of NUP214 for the proliferative effect of the fusion gene.
In order to predict the behavior of these constructs, modeling and simulation of their biomolecular reaction networks are needed to enable the design and fabrication of both the constructs themselves and physical devices based on these constructs.
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