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Values of half lethal concentration (LC50) at 3-day exposure and values of half lethal time (LT50) at 20 IJs/100 μl concentration of both nematodes were compared.
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All the identified CWDE gene families from the five species of plant pathogenic nematodes were compared with each other to identify the gene families which are present in all the species.
In the first comparison gene expression in the resistant Rk plants (incompatible interaction) infected with root-knot nematodes was compared with non-infected Rk plants.
When the response of the two near-isogenic lines infected with nematodes was compared at 9 dpi, it was especially noteworthy that a greater number of genes were suppressed in the resistant genotype than were induced or up-regulated compared to the susceptible genotype.
The turnover diversity displayed high values of dissimilarity when nematode assemblages were compared from different depths (maximal values of 84% between the bathymetric ranges 200 1,000 m and 3,000 4,000 m) and longitudes (greater than 77% comparing Western, Central, and Eastern Mediterranean).
Nematode proteins were compared (BLAST with threshold 1e − 05, 35% identity at over 50% fraction of length) to 257,330 sequences (including multiple chains for a single protein) with known tertiary structure downloaded from the Protein Data Bank (PDB) (Bernstein et al., 1977) to identify homologs.
Two nematode-based tools were compared: (1) nematode-based SQG for estimating the toxic effects based on measured chemical concentrations and (2) the NemaSPEAR index.
Hypoxia of the new isolate and the reference nematode H. bacteriophora were compared.
The highest posterior density (HPD) intervals and means were estimated for the three nematode groups and were compared using a two-tailed randomization test to determine whether posterior rates were different among groups.
Both nematodes were cultured on larvae of the greater wax moth, Galleria mellonella L. (Lepidoptera: Pyralidae) at 25 ± 1 °C.
The same enhanced discovery of sequence conservation was also observed when evolutionarily distant nematode or vertebrate species were compared.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com