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According to our analyses of myosin heavy chain expression, multinucleate myotube formation, and the expression of differentiation-specific genes (myogenin, troponin T, MHC), the differentiation of myoblasts on PCL/PANi nanofibers was strongly dependent on both nanofiber alignment and PANi concentration.
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Finally, the alignment of neurons grown on this substrate was equal to nanofiber alignment and surpassed the alignment of neurites from explants tested in a previous study.
For the two different positioning densities with a controlled 20-μm and 100-μm spacing, respectively, cell spreading in preferential direction could be observed on parallel-aligned nanofibers, and the nanofiber alignment was capable of guiding cell extension, though cell orientation is noticeably less significant for the sparse 100-μm spacing.
Ku et al. invesgated the synergic effects of PCL and PANi blended nanofiber alignment on myoblast differentiations [23].
Neither nanofiber alignment nor PANi concentration influenced cell growth and proliferation, but cell morphology changed significantly from multipolar to bipolar with the anisotropy of nanofibers.
It is observed that nanofiber alignment directs MSC response to physiological loading and that fibroblastic differentiation requires a combination of physiologically-relevant cell material interactions in conjunction with mechanical stimulation.
Immobilized PDGF greatly enhanced the proliferation and tenogenic differentiation of ADSCs; however, nanofiber alignment had no effect.
Using electrically aligned scaffolds, fibroblast directionality deviations can be maintained in the range of nanofiber alignment deviation for fiber sizes down to ∼100 nm.
Nanofiber alignment was quantified using Fast Fourier Transform (FFT) analysis.
Nanofiber alignment was maximal at 30 cm compared to shorter distances.
Yang et al. also examined the effect of nanofiber alignment on the neurite outgrowth of neural stem cells (NSCs) [14].
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