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However, on the BKS background, both mutations produced a life-shortening diabetes, whereas mice on the B6 background were normoglycemic (24– 24).
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The results substantiate that OMVs from a strain with both mutations, produced with a detergent-free process provide better vaccine characteristics than the traditional detergent-based approach.
The trouble, they report in the current issue of Nature, was that each separate cluster of mutations produced a dead receptor, no matter which one was chosen first.
A series of experiments eventually showed that four mutations produced a virus that transmitted well among the animals, he said.
Combining these with field resistance mutations produced a set of 36 non-synonymous polymorphisms associated with drug resistance.
Both mutations produced modest increases in extinction coefficient and quantum yield, and the S179T mutation also accelerated maturation.
The mutations produced an inadvertent stop signal in the gene, causing the cell to make a cut-down version of the gene's normal protein, the researchers report in Nature Genetics.
The new deletion and missense mutations produced an amino acid substitution at positions that are well conserved among several species.
In the first class, the two mutations produced an additive effect.
Furthermore, combining the T157C and L189C mutations produced an ∼500-fold reduction in TPenA sensitivity.
Both mutations produce small amounts of residual enzyme (as in this case), but still at levels that would cause disease.
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