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However, p62 accumulated in both mutants, with higher levels observed in Atg7-deficient muscles (Fig. 6B and C).
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However, both [Lemmel (1985]) and Syed et al. ([2008]) obtained C. acetobutylicum mutants with higher tolerance and productivity using EMS and reported that MNNG was a less effective mutagenic compound.
We obtained several mutants with higher activity, del1 2, del1 3, and S2R.
A truncated version and full-length mutants with higher affinity than the parent sequence are shown.
Mutants with higher kon and koff rates gave rise to higher basal signaling and gene expression but impaired osmoadaptation.
Through multiple selection cycles, in presence of increasing concentrations of the selection pressure, i.e. the toxic compound, mutants with higher tolerance can be selected.
Therefore, the double mutations T166A/E539K and K312E/E539K were designed in the hope of obtaining mutants with higher catalytic efficiency and better thermostability.
The identified mutants with higher oxidative tolerance and catalytic efficiencies would be helpful for further improving the oxidative stability of versatile peroxidase.
According to the positive-selection scheme, we successfully selected l-threonine aldolase mutants with higher activities than the wild-type, from a randomly generated LTA library.
Regarding the genes regulating panicle number per plant, several have been identified through positional cloning from the rice monoculm mutant moc1 and rice dwarf mutants with higher tiller number (Jeon et al. 2011).
Several mutants with higher rates of activities and with different regiospecificities were identified; for example, Escherichia coli TG1 cells expressing either TouA mutant M180T/E284G or E214G/D312N/M399V produce 4-NC 4.5- and 20-fold faster than wild-type ToMO (0.037 and 0.16 nmol/min mg protein from p-NP, respectively).
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