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When cDNAs encoding MWT, Mad10 or Mad12 were expressed in Drosophila S2 cells, we observed that MWT was detectable by phospho-MadCter antibody, while both mutants were not (Figure 4B).
However, both mutants were not significantly different than WT, although lack of NCU08226 caused significantly increased biomass production.
Interestingly however, both mutants were not capable to grow photoheterotrophically when malate was employed as sole carbon source.
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These compounds result in point mutations but not in double strand breaks in DNA and therefore it is not surprising that both mutants are not hypersensitive to these mutagens.
Both oat mutants were not affected in Pro accumulation under stressed or non-stressed conditions.
Wild type Aur-A was degraded by co-transfection of Cdh1, while both ΔN and ΔC Aur-A mutants were not degraded (Figure 2C).
The fittest, most resistant mutants were not always the fastest.
PDGFRA mutants were not responsive to their ligand, PDGFAA, and displayed a high constitutive kinase activity.
In addition, lateral line hair cells in these mutants were not sensitive to prolonged exposure to streptomycin, which is toxic to hair cells.
Interestingly, the mutants were not affected by the mutation and their kinetics was very similar to the wild type strains.
It now appears, however, that these mutants were not capable of hormone-independent growth after all.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com