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Both mutants were identified from a deafness screen as part of a large-scale phenotype-driven mouse ENU mutagenesis programme (8).
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Both the pta1 and ssu72 mutants were identified in the essential gene screen.
Recently, several monogenic root-specific mutants were identified in rice (Table 1).
The equivalent mutants were identified manually, and the execution and analysis were performed automatically by scripts.
Lower: 2 mutants were identified in 96 independent lines transformed the target-1 sgRNA:hSpCas9.
Upper: 14 mutants were identified in 90 independent lines transformed the target-1 sgRNA:pSpCas9.
Replacements of amino acid on beneficial mutants were identified as T66L/D70N, T66V/D70N, E83K, E83H and E83N.
For the target-2 sgRNA-pSpCas9 construct, 94 independent transgenic lines were generated, and 2 mutants were identified (2.1% mutated).
From a small panel of putative inhibitors, compounds that potently and selectively target the inhibitor-sensitized PTPH1 mutants were identified.
Using high throughput screening, more than 20 YqhD mutants were identified to show activity on NADH as a cofactor.
Four unique mutants were identified that maintain the ability to form viral particles, with one showing improved transduction on both 293T and BEAS-2B cells.
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