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In both mutants the amount of polyunsaturated fatty acids was enhanced.
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To determine whether glycolysis is also elevated in xav mutants, the amount of lactate was directly measured and was found to be increased by ∼2.4 fold in xav mutants compared to WT embryos at ∼56 hpf (Figure 5D).
In the flagella of the fla16 and fla17-1 muthets, the amount of IFT122 was maintained at the level similar to wild-type (wt) at either temperature (Fig. 3B, lanes 7 12).
To compare the cleavage efficiency of various CtpB mutants, the amount of 4FAproc substrate was quantified by analyzing the stained gels using the software ImageJ (Schneider et al., 2012 ).
These data indicate that in the genetic background of the mutants, the amount of delocalized protein is not able per se to induce SG recruitment that instead is produced only upon a stress stimulus.
Contrary to a simple scenario predicting impaired interaction in mutants, the amount of precipitated γ-TuC or Plo1 did not appear noticeably different between the wild type and pcp1-15 or pcp1-18 even under stringent high-salt conditions.
In all three mutants, the amounts of flagellar complex B subunits and IFT25 were elevated significantly at both temperatures.
For example, this study showed that in the flagella of the fla15 mutant, the amount of complex B was dramatically increased while complex A disappeared almost completely (Fig. 3).
In the gln1.4 mutant, the amount of malate and other organic acids derived from the TCA cycle such as citramalate and glucarate were reduced.
For each mutant, the amount of Cam2 in eisosomes correlated with the amount of Myo1 in eisosomes and the level of full-length Myo1 detected by immunoblotting (Fig. 1).
Fungal infection eventually led to an apparent acceleration of senescence in a density-dependent fashion in both wild type and mutant, but the amount of chlorosis was greater in the mutant.
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