Both types of mutants should be non-motile; however, the motAB mutant should contain intact, yet non-functional flagella, effectively divorcing the presence of flagella from the process of motility.
Since the likely misunderstandings are highly context dependent, this context should be used to determine which semantic mutants should be produced.
The evidence from ferrets that these viruses are transmissible between mammals by the airborne route, coupled with the demonstrated human virulence of wild-type avian A/H5N1 strains, indicate that H5N1 and its mutants should be taken seriously (21).
Moreover, assuming an incremental testing strategy when combining static and dynamic analysis, this set of mutation operators which generate warning insensible mutants should be used during testing once the chance the faults they modeled be detected during automatic static analysis is reduced.
After zymogram analysis, the CmADH2A homotetramer dramatically decreased in these mutants (Figure S4), which indicated that all the mutants should be ADH2A-deficient.
These mutants should be able to associate with E-Cadherin through the Armadillo repeats, but are known to differ in their signalling ability (Figure 1).
Since one of the cleavage sites remained unchanged, we expected that these V5-MKK4cr and V5-MKK7cr mutantshouldld be still sensitive to LF cleavage.
SΔNA mutants should be of special concern for the poultry industry since they could imply an adaptation of a virus to gallinaceous hosts.
Specifically, the model predicts that the available metabolic energy in the mitochondrial knockout mutants should be ∼50% lower than in WT.
Surprisingly, the TRIM3 deletion constructs ΔRING, ΔRBCC, and RING still resulted in loss of Shank1A protein in COS-7 cells, even though these mutants should be defective as E3 ligases and were incapable of suppressing GKAP protein expression (Figure 3D).
