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Detailed analysis of the culture filtrates of both mutants by using HPLC-MS did not show any candidate masses for possible intermediary structures.
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The International Rice Functional Genomics Consortium IRFGCC) has generated a huge number of gene-indexed mutants by using mutagens such as T-DNA, Tos17 and Ds/dSpm.
Up to the present time, the gTME technique has been used for the construction of new mutant; however in our study we processed a new yeast strain for a library of novel mutants by using SPT15 gene.
In addition, we examined the frequency of abnormal patterning in larval muscles of tw mutants by using the MHC-tauGFP marker to observe live muscles.
Screening of ENU mutants by using fluorescent analogues revealed a mutant with impaired digestive lipid processing.
In addition, levofloxacin MICs were determined for these 2 mutants by using broth microdilution.
Double mutants were obtained from single mutants by using the same primers as for the single mutations.
We performed random mutagenesis and screened for soluble CARD mutants by using a recently developed solubility screen (Harada et al., 2008).
Gene deletions created by the insertion of a region of DNA carrying an antimicrobial drug resistance cassette enable geneticists to select mutants by using bacterial agar into which the relevant drug has been incorporated.
Therefore, we next followed auxin responses during gynoecium and fruit development in wild type and hec1,2,3 mutants by using the pDR5 3xYFP-NLS repDR5 3xYFP-NLSá et al., 2003) to investigate defects in the spatial oreporterion of auxin signalling.
Construction and phenotypic analysis of a sit4 hal3 conditional mutant by using a one-step promoter substitution cassette and the Tet-regulatable dual expression system is also discussed in the chapter.
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