Sentence examples for both mutant and polymorphic from inspiring English sources

In silico analysis of the ATP-bound motor domain simulations showed that deviation of all backbone atoms in the motor domain complex in comparison to the crystal structure was small for both mutant and polymorphic variants compared to WT, with an overall root mean square deviation (RMSD) ranging from 1.2 to 2.7 Å, plateauing after 2 nsec of simulation at 2.4 Å RMSD from the starting structure.

The cells were allowed to express the mutant and polymorphic enzymes for 42 hours and were then fixed by paraformaldehyde.

We have recently performed a thorough characterization of thermal denaturation of holo- and apoproteins by differential scanning calorimetry in AGT WT and eleven mutants and polymorphic variants found in PH1 patients [ 48, 57].

For the WT, mutants, and polymorphic variants, docking against the ensemble of molecular dynamics conformations identified native-like binding poses of ATP close to the previously reported crystal structure of the ATP-bound state.

The transient expression system coupled with flow cytometry to detect intracellular levels of FVIII-based assay would be very useful in studying the intracellular trafficking or wild-type proteins as well as mutants and polymorphic variants of the protein.

To study the effect of these mutations on the secretion of matrilin-3 we cloned and expressed full-length wt, mutant (p.Val194Asp) and polymorphic (p.Glu252Lys) matrilin-3 in mammalian cells.

Western blot analysis also revealed that homogenates of cells transfected with both wild-type HGSNAT and polymorphic mutants, but not of untransfected cells or cells transfected with inactive mutants contained an immunoreactive protein with a molecular mass of ∼48 kDa.

The co-existence of both cryptic and polymorphic species of fig wasps has rendered taxonomy both difficult and error-prone.

Double trouble occurs when both cryptic and polymorphic species co-occur within a species complex, as happens in fig wasps.

This can result in many, sometimes intractable, problems, especially in groups that have both cryptic and polymorphic species.

To evaluate the significance of the TP53 mutation as a molecular marker to predict the prognosis of CRC patients, especially those who received postoperative chemotherapy, we examined the mutation by direct sequencing from fresh CRC tumors and evaluated the R72P polymorphism of the mutated TP53 by a combined mutant allele- and polymorphic allele-specific polymerase chain reaction (PCR).