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Here, we combine both mutagenesis experiments and computational investigations to gain insight into the transfer of ammonia and the mechanism of conduction.
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The functionality of the 10 14 polar amino acids comprising the proton channels is supported by both mutagenesis experiments [20], [24] and bioenergetic analyses [18], and all three channels are highly conserved in most vertebrates [17], [18].
The IBS has been characterized both by mutagenesis experiments and molecular modeling.
Both the mutagenesis experiments and the use of chimeric constructs reveal the strict requirement of three positives charges in the N-terminus for heparan sulfates binding to occur.
Though studies on rice genetics have been documented for over a century, the recent advances in large-scale mutagenesis experiments and sequencing of expressed sequence tags (ESTs), full-length cDNAs, and both the Oryza sativa ssp.
The NT binding site of NTS1 has been mapped by mutagenesis experiments and modeling studies to residues in TM6, TM7 and the extracellular loop 3 [20]–[22].
The proposed receptor positions are then scrutinized by site-directed mutagenesis experiments and measurements of receptor activation by recording intracellular calcium levels following agonist administration.
CM, FB and PNLM performed mutagenesis experiments and protein production.
Where the mutagenesis experiments constituted only a portion of a much larger study, annotation was limited to the mutagenesis experiments and the corresponding wild-type and control experiments.
All mutants were generated by independent mutagenesis experiments and therefore represent unique SNV pools.
TNG carried out the protein expression, purification and immunoblotting studies, performed some cloning and mutagenesis experiments, and performed arginine uptake and decarboxylation assays.
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CEO of Professional Science Editing for Scientists @ prosciediting.com