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Homozygous females of both mouse lines are unable to produce offspring.
However, both mouse lines are unable to increase circulating FA levels during exercise.
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Both mouse lines were maintained on the B6/CBA crossed background.
Both mouse lines were in the C57BL/6J background.
Both mouse lines were backcrossed to the C57/B6 background.
Both mouse lines were housed in the animal facility of the University of Zurich in a 12 h:12 h light dark cycle with access to food and water ad libitum.
With only about 40 milliseconds average duration, USDs of homozygotes of both mouse lines were significantly shorter than those of wild-types and heterozygotes of the respective lines which had an average duration of almost 80 milliseconds (anova with P < 0.05 for the R552H line and P < 0.001 for the S321X line; Fig. 7a,b).
Both mouse lines were engineered to have a point mutation in exon 13 of the mouse Jak2 gene, with JAK2V617F germline expression in a constitutive knock-in (Marty et al., 2010), or in a conditional knock-in activated by breeding the mice with E2A transgenic Cre mice (Mullally et al., 2010).
Interestingly, the two different PSA-deficient mouse lines are both characterized by abnormal motor behaviour (17, 21), which might also be a consequence of reduced autophagy, since the blockage of this process in Atg7-deficient mice leads to major behavioural deficits and widespread inclusion bodies (32).
Second, the costs for many common inbred mouse lines are relatively low in comparison to recombinant inbred lines.
Mouse lines are available upon request.
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