Sentence examples for both morphant and mutant from inspiring English sources

Both morphant and mutant larval zebrafish were sampled at 120 hpf and fixed in 1.5% glutaraldehyde/1% PFA and 70 mM NaPO4, pH 7.2.

This phenotype is specific: it is observed with both morphant and mutant embryos that are deficient in OCRL1, and it is rescued by re-expression of WT OCRL1.

Podocyte effacement was observed in both cofilin-1 morphant and mutant fishes whereas in control fish podocyte foot processes and slit diaphragms were clearly visible (Fig. 1, panel l).

Morphant and mutant both show small eyes with a protruding lens.

In this manuscript we found that cofilin was required for normal glomerular filtration in morphant and mutant zebrafish.

This assay enables us to measure leakiness of the glomerular filtration barrier as a rapid screening tool in individual morphant and mutant fish.

We evaluated zebrafish pronephros function by dextran clearance and structure by TEM in cofilin-1 morphant and mutant zebrafish and we found that cofilin-1 deficiency led to foot process effacement and proteinuria.

To determine if the structural integrity of podocytes was compromised in the cofilin-1 morphant and mutant embryos, we analyzed glomeruli of 120 hpf embryos by transmission electron microscopy.

If a mutant is available in a gene of interest, the morphant and mutant phenotypes should be compared.

Further, the differing observations from various groups may be due to a redundant role for igu/ dzip1 and dzip1-like [ 8, 9], and/or differences between morphant and mutant phenotypes.

We obtained a polyclonal rabbit antibody raised against the first 100 amino acids of zebrafish harmonin (red bar, Fig. 1A), a region common to all predicted isoforms, and probed protein extracts on a western blot to test antibody specificity and to determine the extent of protein depletion in morphant and mutant larvae (Fig. 2B).