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In addition, 13 loci (both monomorphic and polymorphic) possessed species-specific allele size range in A. astacus and A. leptodactylus and can be applied for detecting possible hybrids between these sister species.
Both monomorphic and polymorphic histology may be seen, and monomorphic histology appears to predict an unfavorable prognosis.
This resulted in a set of 64,613 shared loci that contain both monomorphic and polymorphic RAD tags.
The microsatellite loci described here (including both monomorphic and polymorphic primers) can be useful for conservation genetic studies of J. mandshurica.
The application of these microsatellite markers (including both monomorphic and polymorphic loci) will be useful for conservation genetic studies and further investigation of population genetics of P. amurense.
The global success rate (i.e percentage of SNPs successfully genotyped, considering both monomorphic and polymorphic SNPs) obtained for the SNP array developed in the present study was 68.75%.
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A series of ten colorectal and ten breast carcinomas, and their respective lymph node metastases, were examined immunohistologically using monoclonal antibodies (mAb) against both monomorphic and A2 polymorphic determinants, and beta-2-microglobulin (beta 2m).
The two SSR data sets, monomorphic and polymorphic, were further analyzed to investigate a possible relationship between polymorphism level and repeat length, for each SSR type (Table 2).
Schematic of the content of monomorphic and polymorphic markers.
After the calculation of amplification sites, the number of monomorphic and polymorphic markers was depicted using a histogram (Fig. 2).
The global success rate of the assay was 66.9% (considering monomorphic and polymorphic SNPs), and a conversion rate of 51% was achieved (considering only polymorphic SNPs).
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