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Two previous studies from our laboratory have used similar growth conditions to determine iron-responsive genes in C. jejuni using both microarray profiling and RNA-seq [ 2, 7].
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We chose to apply a systems biology approach utilizing both gene expression microarray profiling and corresponding flow cytometry analyses to allow for a comprehensive assessment of the immunopathogenesis of the disease and interaction with the host.
We integrate data sets including microarray profiling and sRNA deep sequencing data from miRNA biogenesis/transport mutants, microarray profiling data of mRNAs in a developmental series, and computational predictions of conserved, genomic stem-loop structures.
The data from the miRNA microarray profiling and primer-extension analysis were generally in agreement except in one instance.
Microarray profiling and real-time PCR analysis revealed a marked down-regulation of miR-206 in nuclear receptor SHP−/− mice.
We further confirmed their identities using comprehensive microarray profiling and Northern blotting.
These data include microarray profiling data and small RNA (sRNA) deep sequencing data derived from miRNA biogenesis/transport mutants, microarray profiling data of mRNAs in a developmental series, and computational predictions of conserved genomic stem-loop structures.
The seminal study of Maret et al. included microarray profiling of both brain and of liver and is, to date, the only study to evaluate genome wide changes in expression in a peripheral organ as a function of sleep state [ 16].
We explore the consistency of RT-PCR expression data with microarray expression by utilizing a dataset where both RT-PCR and microarray profiling data is available for the same miRNA samples.
We performed serial cDNA microarray profiling before, during and after treatment.
After microarray profiling of both HL tumors and cell lines, we found that EBV infection increased the expression of the chemokine CCL20 in both primary Hodgkin and Reed-Sternberg cells and Hodgkin and Reed-Sternberg cell-derived cell lines.
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