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Sequence homology identified 2.541 unique transcripts that are detectable by both microarray hybridizations and 454-sequencing of 1.2 million cDNA tags.
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Both microarray hybridization and next-generation sequencing revealed changes in the products of a miRNA cluster on X chromosome.
Finally, our data showed that 500-600 sinGCs GCs were sufficient to produce enough aRNA to perform both microarray hybridization and qPCR experiments (Table 1).
For the mutant strain, both microarray hybridization and qRT-PCR indicated that so2426 expression levels were not detectable above background, thus providing additional confirmation of deletion of the so2426 gene.
In a recent array screening study, including 20 NSCLC patients and 10 controls, we found miR-21 to be one of the most upregulated miRNAs in tumor tissue, when compared to normal tissue, by both microarray hybridization and quantitative real time polymerase chain reaction (qRT-PCR) technique [ 8].
The integrated data have been used to identify brain, liver and testis-selective genes using a new computational method based on both microarray hybridization intensities and detection calls.
EF performed microarray hybridizations and analysis.
HW performed the microarray hybridizations and scans.
RV: performed microarray hybridizations and scanning.
MP performed microarray hybridizations and genomic sequence data analysis.
MG assisted in microarray hybridizations and data analysis.
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