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Exact(20)
Their expression patterns were confirmed by both microarray and real-time PCR.
The suitability of both, microarray and real-time RT-PCR, for determining the levels of IL-2 mRNA in bovine cells and the good correlation of the two methods has been confirmed in a different context by others [67].
Our results (both microarray and Real-time PCR) showed a similar tendency.
In all studies, the same RNA was used for both microarray and real-time PCR analyses.
The expression trends of all 5 genes were similar in both microarray and real-time PCR analyses.
In this study we observed small gene expression ratios in both microarray and real-time PCR analyses.
Similar(40)
We observed a tendency for upregulation of almost every mitochondrial gene by both microarray and Real Time PCR.
These genes displayed the same expression behavior in both microarray and real time RT-PCR.
Importantly, Pyk2 and several other genes were not significantly different by both microarray and Real-time-PCR, additionally validating both methods.
As an endogenous control, the level of prolyl-tRNA-synthetase gene expression was used to normalize target gene expression levels, since this gene exhibited the least variation in expression across various conditions in both the microarray and real-time PCR experiments.
Compared with the control and saline groups, both miRNA microarray and real-time PCR analyses demonstrated that miR-31, miR-93, miR-100, miR-152, miR-497, miR-192, miR-194 and miR-200b were significantly down-regulated, and miR-122 was highly up-regulated in the hypertonic dialysate group.
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