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The following comparison of brain tissue to the other tissues showed that the differences for both miRNAs were significant (P-adj = 0.003).
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Nine miRNAs were significant in both ANOVA tests after adjusting for multiple comparisons, indicating a significant difference in the expression of these miRNAs across Vonsattel grades (both FDR q-values < 0.05; Additional file 6: Table S6).
Overall, from both the approaches (CC and CO) adopted for the study, eleven miRNAs and four miRNAs were significant for OS and RFS, respectively.
Four miRNAs were significant for OS and were used to construct a risk score.
Of these, four and two miRNAs were significant for Overall Survival (OS) and Recurrence Free Survival (RFS), respectively.
Eleven miRNAs were significant for OS and four miRNAs were significant for RFS in the CO approach as opposed to four and two miRNAs significant for OS and RFS, respectively, in the CC method.
11 miRNAs were significant for OS with permuted p-value ≤ 0.1 and were considered for constructing a risk score.
Two miRNAs were significant for RFS with permutation p value ≤ 0.1 and were used for constructing risk score.
Four miRNAs were significant for RFS with permuted p-value ≤ 0.1 and were considered for constructing a risk score.
The differences of degree distribution between age-related miRNAs and non age-related miRNAs were significant according to the Wilcoxon rank sum test (p < 2.2e-16).
In the CO approach, from 147 miRNAs retained after filtering, 11 and 4 miRNAs were significant for OS and RFS, respectively.
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